FlysisAmp Cells-to-CT 2-Step Probe Kit

 Product Description  

The FlysisAmp Cells-to-CT 2-Step Probe Kit is a complete kit that quickly and efficiently goes from "cells" to "amplification results" and is compatible with 10 - 106 cultured cells. Genomic DNA elimination and RNA acquisition can be completed directly in the cell culture plate. With the high-performance HiScript IV RT SuperMix for first-strand cDNA synthesis and probe-based qPCR mix for amplification, gene expression results can be obtained from cells as fast as 62 min. This kit eliminates the complicated process of traditional extraction, improves efficiency and reduces sample loss in the purification process. This kit is compatible with a wide range of cell types (adherent or suspension cells) and cell quantities, and can also solve the problem of unsatisfactory extraction results with low cell numbers in traditional extraction methods. This kit can be used for high-throughput (96-/384-well cell culture plate) cellular RNA expression analysis.

 Features  

• Fast and simple operation: 62 min to finish from RNA to CT.

• Wide sample compatibility: Compatible with 10 - 10⁶ adherent/suspension cells.

• High throughput: Compatible with different cell culture plates (384/96/24/12/6), RNA acquisition can be done directly in well plates.

• High sensitivity: Combined with the latest hot-start Taq enzyme and optimal buffer system, it avoids missed detections.

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.