EGFP mRNA

 Product Description  

EGFP mRNA expressed enhanced green fluorescent protein (enhanced green fuorescent protein, EGFP), which was initially isolated from jellyfish (Aequorea victoria) with a maximum emission wavelength of 509 nm, and the excited green fluorescence could be easily detected by fluorescence microscopy or flow cytometry. As a reporting mRNA, EGFP mRNA is ideal for monitoring and optimizing transfection efficiency and can be used as a positive control for mRNA transfection.

This mRNA contains high level of Cap1 structure at 5' terminal .Compared with the Cap 0 structure, the mRNA with the Cap 1 structure is more suitable for the mammalian system and has a higher transcription efficiency. Modified UTP and addition of poly(A) tails provide higher transcriptional efficiency and inhibit RNA-mediated innate immune activation while enhancing mRNA stability and longevity in vitro and in vivo.

 Features  

• Ready-to-use & Time-saving: Eliminates time-consuming mRNA preparation—ready for immediate transfection or LNP formulation.

• High capping efficiency: Features Cap 1 structure, optimized for enhanced compatibility in mammalian systems. 

• Incorporation of modified nucleotides and poly(A) tail: Mitigates RNA-induced innate immune activation and improves mRNA stability. 

• High transfection efficiency & Excellent reproducibility: Ensures consistent and reliable experimental results.

 Performance  

Fluorescence images and flow cytometry plots of Vazyme EGFP mRNA expression in HEK 293T cells 20 hours after transfection.

 Components  

 Storage  

Store at -30~-15℃ and transport at ≤0℃.