HiScript III U+ One Step qRT-PCR Probe 5 × Master Mix

 Product Description  

HiScript III U⁺ One Step qRT-PCR Probe 5 × Master Mix is single-tube RT-qPCR premix that is suitable for singleplex or multiplex qPCR detection using RNA (e.g. RNA virus) as template, with extremely high stability. The reaction can be performed directly after adding templates, primers and probes. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.

This kit can prevent the contamination of PCR product. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III U⁺ One Step qRT-PCR Probe 5 × Master Mix can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan). In addition, the dUTP/UDG anti-contamination system is introduced in it, which can work at room temperature to eliminate the influence of amplification product contamination on qPCR and ensure the accuracy of results.

 Features  

• Excellent stability, easy to add samples (one tube mix)

• Excellent balance of high and low concentration template amplification

• Support full premix (except probes and primers)

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤ 0°C.