HiScript III U+ One Step qRT-PCR Probe Kit

 Product Description  

HiScript III U⁺ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit can prevent the contamination of PCR product. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III U⁺ One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III U⁺ One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step U⁺ Mix contains an optimized buffer and dNTP Mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan). In addition, the dUTP/UDG anti-contamination system is introduced in it, which can work at room temperature to eliminate the influence of amplification product contamination on qPCR and ensure the accuracy of results.

 Features  

• Higher detection sensitivity 

• Balanced amplification at high and low template concentrations 

• Applicable to multiplex qPCR 

• Better storage stability 

• Compatible with fast mode

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤ 0°C.