FastPure EndoFree Plasmid Midi Kit

 Product Description  

This kit is applicable for extracting bacterial culture that has been cultured overnight in a volume  of 15 - 50 ml. It uses an optimized alkaline lysis method to lyse cells and utilizes a unique silica  membrane adsorption technique along with special Buffer ERB and Buffer ERW solutions to  specifically bind plasmids and effectively remove impurities such as endotoxins, proteins, and  genomic DNA. Additionally, the unique P2 solution with indicator can indicate the completeness of lysis and neutralization through color changes, ensuring extraction quality and enabling visual  operation. The extracted plasmid DNA has extremely low residual endotoxin levels, making it suitable for various cell transfection and routine operations, including restriction endonuclease digestion, PCR, sequencing, ligation, and other experiments.

 Features  

• Visualization: Intuitive display of neutralization color changes to ensure purity and yield

• Low endotoxin (≤0.1 EU/μg): Ensure downstream experiment success rate >99%

• High yield and high purity: 50 ml high copy bacteria solution, 300 μg plasmid can be obtained

 Components  

 Storage  

Store at room temperature (15 ~ 25°C) and transport at room temperature.

If ambient temperatures often exceed 25°C, we suggest storing RNase A Solution at 2 ~ 8°C.