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HiScript IV All-in-One Ultra RT SuperMix for qPCR
Product Detail
Product Description
HiScript IV All-in-One Ultra RT SuperMix for qPCR (+gDNA wiper) (R433) is a next-generation reverse transcription supermix designed for faster and simpler cDNA synthesis.
Powered by Vazyme’s innovative All-in-One technology, genomic DNA removal and reverse transcription are integrated into a single reaction, eliminating the need for separate DNase treatment or additional reagent handling. The optimized system completes reverse transcription in as little as 5 minutes, enabling a streamlined one-tube, one-step workflow from RNA to qPCR-ready cDNA.Featuring the high-performance HiScript IV Reverse Transcriptase, R433 delivers efficient cDNA synthesis across a broad range of RNA templates, including low-input RNA and challenging templates with complex secondary structures. Combined with effective gDNA removal and a simplified workflow, R433 provides a fast, convenient, and reliable solution for routine and high-throughput gene expression analysis.
Features
True All-in-One Workflow
Integrates gDNA removal and reverse transcription in a single reaction, eliminating separate DNase treatment and simplifying the traditional multi-step RT workflow.
Ultra-Fast 5-Minute Reverse Transcription
Complete reverse transcription in as little as 5 minutes, significantly reducing turnaround time from RNA to qPCR-ready cDNA.
Efficient gDNA Removal
The integrated gDNA removal system effectively minimizes genomic DNA contamination, helping improve the reliability of downstream gene expression analysis.
High-Efficiency cDNA Synthesis
Powered by HiScript IV Reverse Transcriptase with high RNA affinity for efficient and consistent cDNA synthesis across a wide range of RNA inputs.
Excellent Performance with Challenging Templates
Optimized for difficult RNA templates, including GC-rich regions and complex secondary structures, for robust reverse transcription performance.
Built for RT-qPCR
Optimized for downstream real-time PCR applications, providing a streamlined RNA → cDNA → qPCR workflow for routine and high-throughput gene expression studies.
Components
Storage
Storage: -30°C to -15°C
Shipping: Transport at ≤0°C
Performance
Faster Reaction
Complete genomic DNA removal and reverse transcription in 5 minutes.
Total RNA from 293T cells (100 ng) was used as a template for reverse transcription reactions with Vazyme #R433, following the manufacturer’ s protocol at different reverse transcription times (5 min, 10 min, and 15 min). The resulting cDNA was then subjected to qPCR quantification for multiple genes using Vazyme #Q712. The results demonstrate that Vazyme #R433 achieved effective reverse transcription at both the 5-minute rapid time and the 15-minute standard time.
Higher Reverse Transcription Efficiency
Reverse transcription reactions were conducted using 100 ng of RNA from animal cells, plant tissues, and animal tissues as templates with Vazyme #R433, Vazyme #R333, and other commercially available reverse transcription reagents (Supplier A and B), following the recommended protocols for each product. The resulting cDNA underwent quantitative analysis of multiple genes through qPCR. The results indicate that Vazyme #R433 consistently yielded lower CT values across different testing systems, indicating superior reverse transcription efficiency compared to both Vazyme #R333 and the products from Supplier A and B.
Applications
Gene Expression Analysis
For accurate relative quantification of target genes.
High-Throughput Gene Expression Analysis
Ideal for rapid processing of large numbers of RNA samples.
Low-Abundance Transcript Detection
Suitable for sensitive detection of low-abundance RNA targets.
gDNA-Sensitive Applications
Ideal for gene expression assays where genomic DNA contamination may affect quantification accuracy.
Challenging RNA Templates
Suitable for GC-rich transcripts and RNA templates with complex secondary structures.
Broad RNA Sample Compatibility
Compatible with RNA from animal, plant, and cultured cell samples.
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