FastPure Cell/Tissue Total RNA Isolation Kit V2

 Product Description  

The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total  RNA from animal tissues and cells. The kit is based on silica gel membrane purification technology  and does not require β-mercaptoethanol, phenol/chloroform, or any other toxic reagent during  the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure  gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA  Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The  isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can  be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.

 Features  

• Fast: It only requires 6 min at room temperature.

• Safer: No need for β-mercaptoethanol and phenol or chloroform.

• Minimal gDNA residue: No need for on-membrane digestion with DNase I, one-step gDNA contamination removal.

• High RNA purity: High purity of extracted RNA, which is ready for use in demanding downstream experiments.

• Widely applicable: Compatible with all kinds of cell and tissue samples.

 Performance  

• Super Fast

   Obtain one samples in just 6 min at room temperature.

  

• High Quality

  Vazyme #RC112 ('V' in the following figures) and commercially available related products (from suppliers A, B, C, D, E ,F and G respectively) were used to extract total RNA from the rat liver tissue (10 mg) and HEK 293 cell (1×106) samples according to the respective Instructions for Use. Figure A is the agarose gel electrophoretogram of the extracted RNA, while Figures B and C are the statistical charts of RNA concentration and purity results. As shown in the figures, RNA extracted by Vazyme #RC112 has good integrity, high yield and high purity.

  

  
  

• Wide Sample Compatibility

  Vazyme #RC112 was used for the RNA extraction and purification of different samples, and the final RNA product was detected by agarose gel electrophoresis. It shows that Vazyme #RC112 is well compatible in the extraction of RNA from different cell and animal tissue samples.

 Here shows some data from various samples.

 Note: Typical yields can vary due to factors such as species, developmental stage, and growth conditions.

 Components  

 Storage  

Store at 15 ~ 25°C and transport at room temperature.