FastPure Cell/Tissue Total RNA Isolation Kit V3

 Product Description  

This kit enables rapid, high-quality total RNA purification from animal tissues and cells using silica-membrane-based technology, eliminating the need for hazardous reagents like β-mercaptoethanol and phenol-chloroform.

The potent lysis activity of Buffer RL (when combined with Proteinase K) supports compatibility with complex samples and higher input amounts. FastPure columns efficiently remove contaminants and residual gDNA while enabling high-yield RNA binding. With optimized buffers, the resulting RNA is high-purity, free of carryover contaminants, and ready for use in RT-PCR, RT-qPCR, and microarray analysis.

 Features  

• Robust performance on challenging samples: Handles high-protein, high-fiber, and high-fat tissues (muscle, cartilage, fish, adipose tissue) with consistent success rates

• High-Purity RNA for reliable downstream use: Optimized lysis and wash steps eliminate contaminants, ensuring pure RNA for consistent results

• All-in-One compatibility: Works with cells, common tissues, and complex samples to streamline lab workflows and reduce costs

• Fast & safe protocol: Complete RNA extraction in 10 minutes with just two washes—no toxic reagents required

 Performance  

• High Extraction Success Rate

Delivers reliable performance on challenging, difficult-to-lyse samples, with a single kit suitable for all common sample types. RNA was extracted from a variety of animal tissues and cell samples using Vazyme #RC122. Selected purified RNA products were analyzed by agarose gel electrophoresis. The results demonstrate that Vazyme #RC122 exhibits broad compatibility with a wide range of animal tissues and cell samples.

Table 1. RNA yield and recovery from various animal tissues and cell samples

Figure 1. RNA integrity analysis by agarose gel electrophoresis

• Reliable Performance for Downstream Applications: High Yield and Purity

Total RNA was isolated from various animal tissues and cell samples using Vazyme #RC122 and other commercially available total RNA extraction kits, following their respective protocols. The concentration and purity of the purified RNA were then evaluated. As shown in the results, RNA extracted with Vazyme #RC122 yields higher quantities compared to competing products. Additionally, the OD260/230 ratios of RNA isolated with Vazyme #RC122 are closer to the ideal range, indicating superior purity.

Figure 2. Comparison of RNA extraction performance across different kits/suppliers.
(Left) Statistical analysis of RNA extraction yield, shown as recovery percentage (%).
(Right) RNA purity analysis based on the 260/230 absorbance ratio.

• Fast and Safe: Only Two Wash Steps Required

Total RNA can be extracted from various animal tissues and cells in just 10 minutes (the time indicated in the figure corresponds to lysis and centrifugation steps). No toxic reagents such as β-mercaptoethanol or phenol-chloroform are required, ensuring a safer laboratory workflow.

Figure 3. Workflow of the FastPure Cell/Tissue Total RNA Isolation Kit V3

 Components  

 Storage  

Store at 15 ~ 25°C and transport at room temperature.