FreeZol Reagent

 Product Description  

FreeZol Reagent is widely applicable to the extraction of total RNA from cultured cells, animal  tissues, and simple plant tissues. Compared with the conventional Trizol method, this product  features a simple procedure that can be performed at room temperature with no need to use  chloroform for phase separation. In addition, this product ensures the integrity and purity of  the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the  organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be  completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, NGS, and other molecular biology experiments.

 Features  

• Safe and low in toxicity: No need for toxic and harmful reagents such as chloroform and β-mercaptoethanol

• Ease to use: RNA extraction in a monophasic system, no phase separation, all operations at room temperature

• High RNA quality: High purity and integrity of RNA at a yield comparable to the traditional Trizol method

 Performance  

• Superior Quality

  The RNA of rat kidney, spleen, HepG2 and SP2/0 cells were extracted using Vazyme #R711 and other similar products  (Supplier A, Supplier B, and Vazyme #R701), respectively. And the concentration and integrity of RNA products were  analyzed by agarose gel electrophoresis . The results show that the yield and integrity of RNA extracted by  Vazyme #R711 are excellent.

  

• Ultra lysis capability

  129 sample types including cell, tissue, plant,and bacteria, etc. were validated.

  
  

  Cultured Cells

  

  Animal Tissues

  

  Plants 

  

 Components  

 Storage  

Store at 2~8°C and transport them at room temperature.