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This kit is designed for the detection of host cell protein residues in process samples of biologics (such as antibodies, recombinant proteins, vaccines, etc.) expressed in CHO cell lines.
T7 RNA Polymerase (T7 RNA polymerase) is a DNA-dependent 5'→3' RNA polymerase that recognizes the T7 promoter sequence with high specificity ,can be used for transcriptional synthesis of mRNA in IVT.
This kit employs double-antibody sandwich ELISA to detect the residual double-stranded RNA (dsRNA).
In vitro transcription of ssRNA by T7 RNA Polymerase
Gel recovery and crude DNA recovery by one kit
Multi-samples viral DNA/RNA extraction within 11 minutes